Fig. 5: Low leucine levels induced purD expression via NsrP.
a qRT-PCR analysis of the purD expression in the NMEC WT strain in M9 medium supplemented with 0.1 or 5 mM leucine. b qRT-PCR analysis of the purD expression in WT NMEC in LB medium or mouse blood. c Western blot analysis and quantification of PurD-FLAG in WT NMEC in M9 medium supplemented with 0.1 or 5 mM leucine. DnaK was used as the loading control. d–f qRT-PCR analysis of the purD expression in the Δlrp (d), ΔNsrP (e), and NsrPpro-mut (f) strain in M9 medium supplemented with 0.1 or 5 mM leucine. In a–f, data were presented as the means ± SDs (n = 3 independent experiments). ns, nonsignificant. Two-tailed unpaired Student’s t-test was applied.
