Fig. 4: Single-cell type expression in colonic mucosa tissue in inflammatory bowel disease and healthy controls for the genes encoding the 22 candidate proteins.

a A total of nine cell types were identified in colonic mucosa tissue using graph-based clustering by the tSNE. b The t-SNE plot shows the expression of each candidate protein-coding gene across different cell types in the colon mucosa of IBD patients and healthy controls. c The bubble plot shows the expression proportion and levels of each candidate protein-coding gene across different cell types in the colon mucosa of IBD patients and healthy controls. d Cell-specific enrichment of each candidate protein-coding gene. e Comparison of each candidate protein-coding gene expression levels between IBD and health controls in the corresponding specifically enriched cell types. The Wilcoxon rank-sum test was used for analysis. All tests were two sided and adjusted for multiple comparisons. Log2FC > 0.25 and an FDR < 0.05 was considered as significant. The symbols * represent FDR < 0.05, ** represent FDR < 0.01, and *** represent FDR < 0.001. Source data are provided as Supplementary Data 16 and Source Data file.