Fig. 3: MTR4 is dispensable for differentiated spermatogonia mitosis.
From: The nuclear exosome co-factor MTR4 shapes the transcriptome for meiotic initiation
A H&E staining of the sections from Cntl and Mtr4-cKO testes at P8, P9, P10 and P12. Red arrows indicate the representative spermatocytes. P8, n = 2; P9, P10, P11, n = 3 biological repeats. Scale bars, 20 μm. B Schematics diagram of the expression of DDX4, PLZF, STRA8, c-KIT, γH2A.X, and SYCP3 during the early stage of mouse spermatogenesis process. Undiff spg, undifferentiated spermatogonia; A1, type A1 spermatogonia; A2-A4, type A2, type A3 and type A4 spermatogonia, respectively; In-B, intermediate and type B spermatogonia, respectively; pL, preleptotene spermatocyte; L/Z/P/D, leptotene, zygotene, pachytene and diplotene spermatocyte, respectively; MI-MII, meiosis 1 and meiosis 2 spermatocyte metaphase, respectively. C Immunofluorescence staining of STRA8 in P8 Cntl and Mtr4-cKO testis sections. Scale bar, 20 μm. The bar plot shows the quantification of STRA8+ cells per seminiferous tubule. Cntl, n = 47; Mtr4-cKO, n = 48. P-value is based on the two-sided unpaired student’s t test. Error bars, mean ± SEM, n.s. P = 0.5743. D Immunofluorescence staining of DDX4 in P8 Cntl and Mtr4-cKO testis sections. Scale bar, 50 μm. The bar plot shows the population of DDX4+ cells in each seminiferous tubule. Cntl, n = 43; Mtr4-cKO, n = 39. P-value is based on the two-sided unpaired student’s t test. Error bars, mean ± SEM, n.s. P = 0.2921. Source data are provided as a Source Data file.
