Fig. 6: MTR4 deletion leads to upregulation of shorter length and fewer intron genes.

A Violin plot showing the expression changes of genes of varying length in Mtr4-cKO germ cells compared to the corresponding Cntls. n = 3625; 3625; 3625; 3625; 3624 from left to right. P-values are based on the two-sided unpaired Wilcoxon test. Medians are shown in the plot. B Violin plot showing the expression changes of genes with varying intron numbers in Mtr4-cKO germ cells compared to the corresponding Cntls. n = 2436; 4620; 3308; 3992; 3768 from left to right. P-values are based on the two-sided unpaired Wilcoxon test. Medians are shown in the plot. C Violin plot showing length of genes exhibiting upregulated, no-changed and downregulated expression. Up, n = 1145; n/c, n = 14,175; down, n = 2805. P-values are based on the two-sided unpaired Wilcoxon test. n: the number of differential expression genes. D Violin plot showing the intron number of genes exhibiting upregulated, no-changed and downregulated expression. Up, n = 651; n/c, n = 12,332; down, n = 2705. P-values are based on the two-sided unpaired Wilcoxon test. n: the number of differential expression genes. E Violin plot showing the length of upregulated meiotic genes and downregulated mitotic genes in Mtr4-cKO germ cells. Upregulated meiotic gene, n = 211; downregulated mitotic genes, n = 1389. P-value is based on the two-sided unpaired Wilcoxon test. F Violin plot showing intron number of upregulated meiotic genes and downregulated mitotic genes in Mtr4-cKO germ cells. Upregulated meiotic gene, n = 164; downregulated mitotic genes, n = 1,83. P-value is based on the two-sided unpaired Wilcoxon test. G Violin plot showing the length of specifically expressed genes at individual spermatogenic stages in sc-RNA seq dataset. BG2M, n = 1509; G1, n = 60. P-value is based on the two-sided unpaired Wilcoxon test. H Violin plot showing the intron number of specifically expressed genes at individual spermatogenic stages in sc-RNA seq dataset. BG2M, n = 1509; G1, n = 60. P-value is based on the two-sided unpaired Wilcoxon test. I Cumulative distribution of gene length of specifically expressed genes at BG2M and G1 stage. BG2M, n = 1509; G1, n = 60. P-value is based on the two-sided unpaired Wilcoxon test. J Cumulative distribution of intron number of specifically expressed genes at BG2M and G1 stage. BG2M, n = 1509; G1, n = 60. P-value is based on the two-sided unpaired Wilcoxon test. K Violin plots showing the expression level of Mtr4 (left) and Dis3 (right) at individual spermatogenic stages from sc-RNA seq dataset. BG2M, n = 58; G1, n = 204. P-values are based on the two-sided unpaired Wilcoxon test. L The expression of Mtr4/exosome reduces and the meiotic genes with shorter length and fewer introns activate during mitotic-meiotic transition. Data in (A–H, K) are presented as box and whisker plots. The central lines denote median value, while the bounds of box mark the 25th to 75th percentiles and whiskers refer to minimum and maxima values. Source data are provided as a Source Data file.