Fig. 3: Phylogenetic profile of AArfs.

A maximum likelihood phylogenetic tree is shown at the center. The tree was inferred in IQ-TREE with LG + R5 model automatically selected. Brach support was evaluated by ultrafast bootstrap algorithm (1000 replicates). Each AArf protein is also denoted by a bar surrounding the tree, with bar length proportional to protein length, colored by species lineage. Predicted structures of select AArfs are shown close to the corresponding bars. Cyan, core Arf GTPase structure; gray, additional non-Arf domains present in certain AArf lineages. Stars denote AArfs experimentally tested in yeast; red stars, those that displayed prominent membrane association; green starts, the rest.