Fig. 4: AArfs are potential hubs in a protein network functioning in organelle dynamics. | Nature Communications

Fig. 4: AArfs are potential hubs in a protein network functioning in organelle dynamics.

From: Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis

Fig. 4

a AArfs can be regulated by Arf GEFs. Overexpression of several Arf GEFs altered the subcellular distribution of AArfs. Shown are representative live cell fluorescent microscopy images of yeast cells expressing Heim12F4_9-5-GFP or GerdYT_6_3-5-GFP before and 4 h after galactose-induced GEF overexpression. Fluorescent intensity profiles of cropped areas are presented at the side, with positions of plasma membrane marked by red arrows. Schematic AArf subcellular distribution patterns are shown at the bottom. b, c AArfs can interact with a protein network regulating organelle dynamics. b GO term enrichment in GerdYT_6_3-5 interactome in yeast. Membrane and organelle related terms are colored blue. Data analyzed by two-sided t-test with no adjustment for multiple comparisons. Significance threshold (s0), 0; false discovery rate (FDR), 0.05. c A sub-network of GerdYT_6_3-5 interacting proteins, consisting of those mediating intracellular protein transport, organelle function, and lipid metabolism. Lines and dashed lines denote known protein-protein interactions in STRNG. White scale bar, 2 μm.

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