Fig. 1: Nedd4-1(NE) is localized to late endosomes. | Nature Communications

Fig. 1: Nedd4-1(NE) is localized to late endosomes.

From: The primate-specific Nedd4-1(NE) localizes to late endosomes in response to amino acids to suppress autophagy

Fig. 1

A Schematic representation of the canonical Nedd4-1 and the Nedd4-1(NE) isoform. Due to alternative splicing, Nedd4-1(NE) contains a 516-amino acid NE region and only a small part of the C2 domain. Images are drawn to scale. B–E HeLa cells were transfected with Flag-tagged constructs, as indicated. Cells were fixed, permeabilized, and immunostained with anti-Flag antibodies (green). Nuclei were stained with DAPI (blue). F HeLa cells were treated as in (B), and fixed samples were additionally immunostained using antibodies against endogenous Rab7 (red). G Quantification of the percentage of overlap of Nedd4-1(NE) with various endogenous protein markers. Data are means ± SD (N = 3 independent experiments, n = 27-36 cells/experiment; precise values are indicated in the Source Data file). All scale bars are 10 μm.

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