Fig. 6: Seasonal tissue remodeling in mouse heart, pancreas, and kidney.
From: Non-human primate seasonal transcriptome atlas reveals seasonal changes in physiology and diseases
a Effects of 1-month exposure to short-day and cool (SC) or long-day and warm (LW) conditions on organ weight in CBA/N and C57BL/6 N mice. Numbers within the graph indicate the P value (unpaired two-tailed t-test, mean + SEM, n = 8). b (top) Effects of 1-week exposure to SC or LW conditions on Ki67-positive cell numbers in the heart (HEA), pancreas (PAN), and kidney (KID) of C57BL/N mice. Numbers within the graph indicate the P value (unpaired two-tailed t-test, mean + SEM, n = 4). (bottom) Effects of 1-week exposure to SC or LW conditions on cardiomyocyte diameter, pancreatic acinar cell size, and glomerular size. (t-test, mean + SEM, n = 4). c Representative images of Ki67 immunohistochemistry in the heart (HEA), pancreas (PAN), and kidney (KID) under SC and LW conditions. The number of Ki67 immuno-positive cells (red arrows) was higher under SC than LW conditions in the HEA and PAN, while glomerular size was larger under SC than LW conditions in the KID. d Representative images for the H&E staining of cardiomyocytes under SC and LW conditions. e Representative images of the immunohistochemistry of β-catenin, which localizes to the acinar cell membrane. Note that the nuclear density (i.e., number of nuclei in the field) is greater under LW than SC conditions, indicating a larger cytoplasmic area under SC conditions. L: Islet of Langerhans; G: Glomerulus. Scale bars: 50 μm. f Daily food and water intake in male C57BL/6 N mice kept under SC and LW conditions. Numbers within the graph indicate the P value (unpaired two-tailed t-test, mean + SEM, n = 6).
