Fig. 4: Foxk1 and Foxk2 improve cardiac regeneration and repair in adult mice.
From: Foxk1 and Foxk2 promote cardiomyocyte proliferation and heart regeneration
A Experimental design: AAV9-GFP, AAV9-Foxk1, or AAV9-Foxk2 was injected into adult mice via the tail vein after MI. Echocardiography was performed 1 day after MI (dpi), 7 dpi, 14 dpi, 28 dpi, 42 dpi, and 56 dpi. Heart tissue was collected at 14 dpi and 56 dpi. B Western blot analysis of FOXK1 and FOXK2 levels in mouse hearts infected with AAV9-GFP, AAV9-Foxk1, or AAV9-Foxk2 at 14 dpi (n = 3 per group). C–E Echocardiography analysis of heart function in mice administered AAV9-GFP, AAV9-Foxk1, or AAV9-Foxk2 at the indicated time points and quantification of EF and FS (n = 5 in sham group and n = 8 in MI group). F WGA staining of Foxk1- or Foxk2-overexpressing hearts at 56 dpi (n = 4 in sham group, n = 6 in AAV9-GFP group, n = 4 in AAV9-Foxk1 group, n = 5 in AAV9-Foxk2 group). G Masson staining and quantification of fibrotic scars at 56 dpi after LAD ligation and AAV9-GFP, AAV9-Foxk1 or AAV9-Foxk2 treatment (n = 4 in sham group, n = 8 in MI group). Data are presented as mean ± SEM. p values were determined by two-way ANOVA with Bonferroni multiple comparisons test (D, E) and one-way ANOVA with Bonferroni multiple comparisons test (F, G). Source data are provided as a Source Data file.
