Fig. 2: HiPMs promote human cardiomyocyte proliferation. | Nature Communications

Fig. 2: HiPMs promote human cardiomyocyte proliferation.

From: Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration

Fig. 2

a Schematic diagram depicting the co-culture of human cardiomyocytes and hiPMs in 2D and 3D culture systems. Cartoon elements used in the schematic diagram were created using BioRender. b Images showing co-culturing of human cardiomyocytes and hiPMs by brightfield microscopy and immunofluorescence microscopy. Human cardiomyocytes co-cultured with hiPMs (+hiPM) were compared with human cardiomyocytes alone (-hiPM). For immunofluorescence images, human cardiomyocytes were labeled with cardiac Troponin T (cTnT) (red), hiPMs were labeled with CD14 (green), and all the nuclei were counterstained with Hoechst (blue). Scale bars, 100 μm for brightfield images and 10 μm for immunofluorescence images. The experiment was repeated three times independently with similar results. c Representative immunofluorescence images and quantification of Ki67-positive human cardiomyocytes co-cultured with hiPMs in a 2D culture system. Human cardiomyocytes were labeled with cTnT (red) and Ki67 (green); cardiomyocyte nuclei were counterstained with Hoechst (blue). Scale bar, 10 μm. n = 3 biological repeats per group. d Brightfield images showing the co-culturing of human cardiomyocytes and hiPMs in 3D culture system. Human cardiomyocytes co-cultured with hiPMs (+hiPM) were compared with human cardiomyocytes alone (-hiPM) at 10 days after the start of 3D culturing in Ultralow attachment 96 U-well plates. Scale bar, 100 μm. The experiment was repeated three times independently with similar results. e Representative immunofluorescence images and quantification of Ki67-positive human cardiomyocytes co-cultured with hiPMs in the 3D culture system. Human cardiomyocytes were labeled with cTnT (red) and Ki67 (green); cardiomyocyte nuclei were counterstained with Hoechst (blue). Scale bar, 15 μm. n = 3 biological repeats per group. Quantitative data are presented as the mean ± SEM. Groups were compared using a two-tailed unpaired Student’s t test (c and e). Source data are provided as a Source Data file.

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