Fig. 1: High expression of SIRT7 is positively associated with endometrial cancer progression.
A SIRT7 expression analysis of normal endometrium tissue (n = 35) and endometrial cancer tissue (n = 544), data from TCGA. B SIRT7 expression analysis of endometrial cancer in grade 1–2 and high grade, data from TCGA (left, G1/G2 n = 218, High Grade n = 326), data from GSE115810 (right, G1/G2 n = 18, High Grade n = 6). C SIRT7 expression analysis of human EC tissue by IHC. Endometrial cancer and paired para-tumor tissues (tumor n = 21, para-tumor n = 18) were stained with SIRT7 and quantified by IHC score. Scale bar = 50 μm. D SIRT7 expression analysis by Western blot. Tissues of paired endometrial cancer with adjacent normal tissue (n = 10) were analyzed for SIRT7 expression and quantified. The relative expression level was calculated by SIRT7/GAPDH. ImageJ software was used. E SIRT7 expression analysis of human EC tissue by IHC. Primary tumor tissue of EC and paired metastatic tissues in ovary or fallopian tube (n = 13) were stained with SIRT7 and quantified by IHC score. Scale bar = 50 μm. F SIRT7 expression analysis of human EC tissue by IHC. Primary tumor tissue of EC and paired metastasis tissues in lymph nodes (n = 15) were stained with SIRT7 and quantified by IHC score. Scale bar = 50 μm. G The diagram of Lkb1f/f mice crossed with Pgr-Cre mice. H The representative photograph, H&E staining and immunohistochemistry staining of uterus tissue of Lkb1 CKO (Lkb1f/f Pgr-Cre) mice and control mice (Lkb1f/f) at different weeks post birth (n = 5). Scale bar = 50 μm. I Western blot analysis of the uterus tissue of Lkb1 CKO mice and control mice at six weeks post birth. Different EC tumor nodules of Lkb1 CKO mice were isolated and analyzed (n = 6). Relative Sirt7 expression was quantified by Sirt7/Tubulin. The P value was calculated with two tailed unpaired t test for (A–C, I) and calculated with two tailed paired t test for (D–F). Data are presented as mean values ± s.e.m.
