Fig. 7: Estrogen acts as the upstream regulator of SIRT7-PTEN axis. | Nature Communications

Fig. 7: Estrogen acts as the upstream regulator of SIRT7-PTEN axis.

From: SIRT7 facilitates endometrial cancer progression by regulating PTEN stability in an estrogen-dependent manner

Fig. 7

A Immunoblots showing PTEN level after the cells being treated with 17 beta-Estradiol (E2) for 48 hours with indicated concentration in HEC-1B cells. B Immunoblots showing PTEN level after estrogen treatment for 48 hours with indicated concentration in HEC-1B cells with or without SIRT7 knockdown. C Representative pictures and quantitative analysis of PLA assay demonstrating the interaction of endogenous PTEN and SIRT7 protein with or without E2 stimulation. HEC-1B cells were treated with E2 (100 nM, 48 h) and MG132 (10 μM, 6 h) as indicated. 100 cells were quantified. Scale bar = 100 μm. D Co-IP assay manifesting the interaction between endogenous SIRT7 and PTEN in HEC-1B cells with E2 stimulation for 48 hours with indicated concentration. E Co-IP assay manifesting the interaction between SIRT7 and PTEN in HEK293FT with E2 stimulation for 48 hours with indicated concentration. F Co-IP assay manifesting the interaction between SIRT7 and PTEN after ESR1 overexpression in HEK293FT cells. G The acetylation level of PTEN in HEK293FT cells with stimulation of E2 (50 nM, 48 h) with or without SIRT7 overexpression. H The AcK260 level of endogenous PTEN in HEC-1B cells with or without stimulation of E2 (100 nM, 48 h) detected by AcK260-PTEN antibody. I Co-IP assay manifesting the interaction between endogenous NEDD4L and PTEN in HEK293FT with SIRT7 overexpression and E2 stimulation as indicated in HEK293FT cells. J The ubiquitination level of PTEN in HEK293FT cells with stimulation of E2 of indicated concentration. Cells were treated with MG132 (10 μm) for 6 hours before lysis. AcK260-PTEN (K) and SIRT7 (L) analysis of human uterus endometrium tissue during menstrual cycle by IHC. Endometrium in secretory phase (n = 12) and proliferative phase (n = 17) were stained with AcK260-PTEN and SIRT7 and quantified by IHC score. Scale bar = 50 μm. M Schematic diagram showing the mechanisms that SIRT7 upregulation facilitates EC progression through PTEN-K260 deacetylation and subsequent ubiquitination and degradation under long-term stimulation of estrogen. Created in BioRender. Hu, Z. (2025) https://BioRender.com/d55g597. The P value was calculated with two tailed unpaired t test for (C, K, L). Data are presented as mean values ± s.e.m.

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