Fig. 3: Selective neurochemical sensing from CCMs.
From: Stabilized carbon coating on microelectrodes for scalable and interoperable neurotransmitter sensing
Concentration-dependent response of CCMs to (a) serotonin (5-HT), (b) epinephrine (EP), and (c) adenosine (ADE). The ADE was detected with an extended 1.5 V switching potential. The main oxidation peak potentials are, 5-HT: 0.40 and 0.64 V; EP: 0.62 V; ADE: 1.10 and 1.37 V. d Simultaneous detection of DA and ADE. Top: Background-subtracted voltammogram collected at the time indicated by the vertical dash line; Bottom: A color plot serially displaying all background-subtracted voltammograms. 100 μL PBS containing 2 μM DA and 10 μM ADE was introduced to the surface of a CCM. e Jackson waveform for 5-HT detection. Top: Jackson waveform with a scan rate of 1000 V/s; Left: Background-subtracted voltammogram in the presence of 1 nM 5-HT to demonstrate a low limit of detection; Right: A comparison of the background-subtracted voltammograms in the presence of 500 nM DA and 100 nM 5-HT to show its selective sensing of 5-HT over DA. The highlighted portions with corresponding colors in the molecular structure in (a–c) indicate the electrochemical active groups. Except for the Jackson waveform, a triangle waveform with a scan rate of 400 V/s was applied to CCMs. All waveforms were repeated at 10 Hz. All tests were performed in PBS (pH 7.4).
