Fig. 1: KLF10 is upregulated in mature adipocytes in response to exercise and fasting. | Nature Communications

Fig. 1: KLF10 is upregulated in mature adipocytes in response to exercise and fasting.

From: Exercise-induced anti-obesity effects in male mice generated by a FOXO1-KLF10 reinforcing loop promoting adipose lipolysis

Fig. 1

From a–h Chow diet-fed 8-week-old male mice were subjected to treadmill training for 8 weeks before being sacrificed for analysis. Sed, non-exercise. Ex, exercise. a, b Messenger RNA (mRNA) levels of Klf10 in stromal vascular fraction (SVF) and mature adipocytes of inguinal white adipose tissue (iWAT), respectively. c Representative western blotting of iWAT-isolated mature adipocytes lysates. β-tubulin serves as an internal control. d, e The mRNA levels of Klf10 in SVF and mature adipocytes of epididymal white adipose tissue (eWAT), respectively. f Representative western blotting of eWAT-isolated mature adipocytes lysates. g, h The mRNA levels of Klf10 in SVF and mature adipocytes of brown adipose tissue (BAT), respectively. i–n Chow diet-fed 8-week-old male mice were fasted for 12 h before being sacrificed for analysis. i, j The mRNA levels of Klf10 in SVF and mature adipocytes of iWAT, respectively. k, l The mRNA levels of Klf10 in SVF and mature adipocytes of eWAT, respectively. m, n, The mRNA levels of Klf10 in SVF and mature adipocytes of BAT, respectively. For statistical analysis, unpaired two-tailed Student’s t tests were performed. n = 6 male mice per group in all the above experiments. All data show the means ± SD. Source data are provided as a Source Data file.

Back to article page