Fig. 3: Adipocyte-specific knockout of KLF10 (KLF10AKO) impairs exercise-induced browning and lipid catabolism in male mice.
Chow diet (CD) fed KLF10AKO and wild type (WT) control male mice were subjected to treadmill training (Ex) or kept sedentary lifestyle (Sed) for 8 weeks before being sacrificed for analysis. a The treadmill exercise program is illustrated. The graph model was created in BioRender. Zhu, J. (2025) https://BioRender.com/z12f220. b Representative images of hematoxylin and eosin (H&E) staining of adipose tissues. Scale bars, 100 μm. Quantification of adipocyte diameter in iWAT and eWAT was shown below. c Immunohistochemistry (IHC) staining of UCP1 in iWAT and BAT. Scale bars, 50 μm. d The mRNA levels of the indicated genes in iWAT. Data were normalized to the WT/Sed group. e, f After 7 weeks of exercise training, the serum free fatty acid (FFA) and glycerol levels of CD-fed mice under 22 °C or 4 °C condition for 12 h. g The mRNA levels of the indicated genes in iWAT and eWAT. Data were normalized to the WT/Sed group. h, i Representative western blotting of iWAT and eWAT lysates. β-tubulin serves as an internal control. For statistical analysis, two-way ANOVA plus Tukey’s post hoc tests were performed in b, d, g; three-way ANOVA plus Tukey’s post hoc tests were performed in e, f. n = 6 male mice per group in all the above experiments. All data show the means ± SD. Source data are provided as a Source Data file.
