Fig. 4: KLF10^AKO attenuates the effects of exercise on resisting diet-induced obesity (DIO) and metabolic disorders in male mice.

The 12-week high-fat diet (HFD)-fed KLF10^AKO and WT control male mice were subjected to treadmill training (Ex) or kept sedentary lifestyle (Sed) in the last 10 weeks of HFD feeding before being sacrificed for analysis. a–e Body weight, body weight gain, body composition and adipose tissue weight of the mice, respectively. f Representative images of hematoxylin and eosin (H&E) staining of adipose tissues. Scale bars, 100 μm. g Cold tolerance test was performed at the 10th week of HFD feeding. After 10 h of fasting, mice were subjected to cold exposure (4 °C) in the fasted state for 5 h, and the rectal temperatures of the mice were measured. h–j Fasting blood glucose, fasting plasma insulin and HOMA-IR of the mice, respectively. k The glucose tolerance test (GTT) were performed in mice after 11 weeks of HFD feeding. l Analysis of the GTT data in m, with subtraction of the basal glucose to generate an area of the curve (AOC). m The oxygen consumption rate of iWAT of mice were measured. n, o Regression-based analysis of absolute VO₂ and VCO₂ against body weight of mice, respectively. p The mRNA levels of indicated genes in iWAT of mice. Data normalized to the WT/Sed group. For statistical analysis, two-way ANOVA plus Tukey’s post hoc tests were performed in a–e, g–j, l, m, p; two-sided analysis of covariance (ANCOVA) was performed in n, o. n = 6 male mice per group in all the above experiments. All data show the means ± SD. Source data are provided as a Source Data file.