Fig. 6: KLF10^AKO impairs lipolysis to exacerbate diet-induced obesity (DIO) and metabolic disorders in male mice.

KLF10^AKO and WT control male mice were subjected to injection of adeno-associated viral (AAV) vectors harboring the genes encoding ATGL (AAV-ATGL), HSL(AAV-HSL) or GFP (AAV-GFP, as the control) in iWATs and eWATs. One week later, mice were fed with high-fat diet (HFD) for 12 weeks before being sacrificed. a Cold tolerance test was performed at the 10th week of HFD feeding. After 10 h of fasting, mice were subjected to cold exposure (4 °C) in the fasted state for 5 h, and the rectal temperatures of the mice were measured. b–d, Body weight, body weight gain and adipose tissue weight of the mice, respectively. e Representative images of hematoxylin and eosin (H&E) staining of mice adipose tissues. Scale bars, 100 μm. Quantification of adipocyte diameter in iWAT and eWAT was shown below. f, g The serum FFA and glycerol levels of 10-week HFD-fed mice under basal or Cl316,243 stimulated condition (injection with 1 mg/kg body weight). h GTT was performed in mice after 11 weeks of HFD feeding. i Analysis of the GTT data in h, with subtraction of the basal glucose to generate an area of the curve (AOC). j ITT was performed in mice after 11 weeks of HFD feeding. k Analysis of the GTT data in j, with subtraction of the basal glucose to generate an area of the curve (AOC). l HOMA-IR of mice after 12 weeks of HFD feeding. For statistical analysis, two-way ANOVA plus Tukey’s post hoc tests were performed in a–e, i, l; three-way ANOVA plus Tukey’s post hoc tests were performed in f, g; Kruskal–Wallis test with Dunn’s correction was performed in k. n = 6 male mice per group in all the above experiments. All data show the means ± SD. Source data are provided as a Source Data file.