Fig. 8: Adipose-specific KLF10 transgenic (KLF10^ATG) promotes exercise-mediated alleviation of DIO and metabolic dysfunction in male mice.

The 12-week high-fat diet (HFD)-fed KLF10^ATG and WT control male mice were subjected to treadmill training or kept sedentary lifestyle in the last 10 weeks of HFD feeding before being sacrificed for analysis. a–e Body weight, body weight gain, body composition and adipose tissue weight of the mice. f Representative images of hematoxylin and eosin (H&E) staining of adipose tissues. Scale bars, 100 μm. g, h Quantification of adipocyte diameter in iWAT and eWAT based on the results of f. i Cold tolerance test was performed at the 10^th week of HFD feeding. After 10 h of fasting, mice were subjected to cold exposure (4 °C) in the fasted state for 5 h, and the rectal temperatures of the mice were measured. j GTT were performed in mice after 11 weeks of HFD feeding. k Analysis of the GTT data in j, with subtraction of the basal glucose to generate an area of the curve (AOC). l HOMA-IR of the mice. m, n Regression-based analysis of absolute VO₂ and VCO₂ against body weight of mice. o FFA release in iWAT isolated from mice under basal or CL316,243 (2 μM) stimulated conditions. p The serum FFA level of HFD-fed mice under basal or Cl316,243 stimulated condition (injection with 1 mg/kg body weight). q The mRNA levels of indicate genes in iWAT and eWAT of mice. Data normalized to the WT/Sed group. r Representative western blotting of iWAT lysates. s The mRNA levels of indicate genes in iWAT of mice. Data normalized to the WT/Sed group. For statistical analysis, two-way ANOVA plus Tukey’s post hoc tests were performed in a–e, g–i, k, l, q, s; two-sided analysis of covariance (ANCOVA) was performed in m, n; three-way ANOVA plus Tukey’s post hoc tests were performed in o, p. n = 6 male mice per group in all the above experiments. All data show the means ± SD. Source data are provided as a Source Data file.