Fig. 3: Liposomal LNP NanoLuc mRNA system exhibits a similar protein BMC profile but reduced total adsorbed protein compared to LNP with Onpattro-like lipid compositions.
From: Liposomal lipid nanoparticles for extrahepatic delivery of mRNA
Liposomal LNP (nor-MC3/ ESM: cholesterol/ PEG-DMG; 20/40:40/1.5 mol/mol; RB/I = 4) and LNP with the Onpattro-like composition (MC3/DSPC/cholesterol/DMG-PEG: 50/10/38.5/1.5 mol/mol), N/P ratio of 6, were formulated, incubated with plasma and isolated as indicated in Methods. A Protein to lipid ratio (wt/wt, µg/µg), total protein measured from a BCA assay, total lipid calculated from DiD-C18 absorbance measurement (mean ± S.D., n = 3). Data were analyzed through a two-tailed unpaired t-test, *p = 0.0296. as indicated in Methods. B SDS–PAGE of the plasma proteins adsorbed to the surface of Onpattro-like and liposomal LNP at 1 hour post incubation (mean ± S.D., n = 3). The plasma protein profile is also shown. C Heatmap analysis of the > 1000 proteins identified (iBAQ intensity log) (White-orange indicates the highest abundance while purple-black indicates lower abundance). D The proportions of proteins with distinct biological functions in the protein coronas of LNP mRNA systems with the Onpattro-like lipid composition as compared to the liposomal LNP (Grey: Others; Yellow: Immuno Proteins; Blue: Apolipoprotein; Green: Complement Proteins; Pink: Albumin; Purple: Coagulation Factors). Source data are provided as a Source Data file.
