Fig. 2: Cytotoxicity and colony formation assays of 70 nm Au-based NPs.
From: Redox disruption using electroactive liposome coated gold nanoparticles for cancer therapy
a A variety of cancer cell lines, including Hep G2 hepatoma cells, HA22T hepatoma cells, and MDA-MB-231 triple-negative breast cancer cells, along with the normal cell lines NeHepLxHT hepatocytes and M10 breast epithelial cells, were chosen for detailed cytotoxicity studies. Representative images illustrate the distribution of live and dead cells in NeHepLxHT, M10, Hep G2, HA22T, and MDA-MB-231 cells following a 72-h treatment with 70 nm Au@PEG, Au@Lipo, Au@E. coli, and Au@MIL NPs at a concentration of 300 ppm Au. Nuclei, live cells, and dead cells were stained with Hoechst 33342 (blue), calcein AM (green), and EthD-1 (red), respectively. These images are representative of three independent experiments. Scale bars, 100 μm. b Colony assay of 70 nm Au-based NPs in NeHepLxHT, M10, Hep G2, HA22T, and MDA-MB-231 cells (n = 3, a repeated experiment). Cells were incubated with 300 ppm of Au-based NPs for 72 h and maintained without them for another 14 days. The formation of clones was fixed and stained with 0.5% crystal violet, and the results were measured using ImageJ software. Data were presented as the mean ± SD, and p-values were calculated using one-way ANOVA. Source data are provided as a Source Data file.
