Fig. 4: Conjugated cholic acids hydrolysis across bacterial strains and wild-type mice supplemented 50 mg/kg TCA or GluCA via peanut butter pellets self-administrated method under DSS-induced colitis.
From: Balance between bile acid conjugation and hydrolysis activity can alter outcomes of gut inflammation
a The abundances of conjugated cholic acids are represented as colors corresponding to log10transformed peak areas (area under the curve, AUC). Values represent the mean from n = 3 replicates of log10 areas changes compare to medium after 48 h in vitro culture. Phylogenetic tree of 17 Bacteroidetes strains using GTDB-tk based on whole bacteria genome. b Experimental animal design in wild-type C57BL/6 J mice fed with a final dose of 50 mg/kg TCA or GluCA peanut butter pellets under DSS-induced colitis (n = 10 each group). c–e Relative abundance of CA, DCA and oxocholic acid abundance in feces. Fecal metabolites data was analyzed by Kruskal-Wallis followed by Dunn’s post hoc, and *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. All boxplots are the interquartile range, center line is the median, with the whiskers denoting minima and maxima.
