Fig. 1: Workflow for the identification of dsRBPs by PISA assay in this study. | Nature Communications

Fig. 1: Workflow for the identification of dsRBPs by PISA assay in this study.

From: Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator

Fig. 1

The HeLa cells were disrupted using PBS with four freeze-thaw cycles. The extracts were incubated with poly(I:C) or the same volume of PBS for 30 min at RT and divided into 12 aliquots, each heated at the specified temperatures. Following centrifugation, the supernatants were combined into four tubes according to the experimental design. Four biological replicates were conducted. After digestion with trypsin, the peptide samples were analyzed by LC-MS/MS. ΔSm was measured as the difference in integral abundances of the protein between the poly(I:C) treated and untreated samples.

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