Fig. 2: The identification of dsRBPs via the PISA assay.

a Volcano plots represent the quantitative analysis of proteins across four biological replicates in four PISA assays. Proteins with significantly increased soluble amount are shown as red dots, while proteins with decreased soluble amount are displayed as blue dots. Log₂FC was plotted on the x-axis and −log₁₀ P value was plotted on the y-axis. P values were calculated by an unpaired two-sided t-test with no adjustment. PISA proteome integral solubility alteration. FC fold change. Molecular function (b) and biological process (c) analysis of proteins with increased solubility identified by PISA, with P < 0.05 as cutoff and the top 15 terms are displayed. The P values were calculated using the hypergeometric distribution. d InterPro-annotated domain enrichment analysis with P < 0.05 as the cutoff. The P values were calculated using the hypergeometric distribution. e A scatter plot displaying protein abundance changes incubated with or without poly(I:C) at 37 ^oC (n = 2 independent experiments). Proteins with |log₂FC| > 0.58 are shown in blue (decreased) and red (increased). Ctrl, control. f Molecular function analysis of proteins with increased solubility with the top 10 terms displayed. The P values were calculated using the hypergeometric distribution.