Fig. 7: ZNF385A interacted with splicing factors.

a The workflow of TurboID proximity labeling for identifying ZNF385A-interacting proteins. b Western blotting validation of TurboID-based proximity labeling. Results are representative of two independent experiments. c Comparison of the significantly enriched proteins from ZNF385A vs WT, ZNF385A vs NLS, and ZNF385A vs NES groups shown by a Venn plot. WT, wild type. NLS, nuclear localization signal. NES, nuclear export signal. ID, identity. FC, fold change. d Anti-FLAG co-IP assay was performed to validate the binding of PTBP1 and TARDBP to ZNF385A. EV, empty vector. Results are representative of two independent experiments. e Anti-PTBP1 co-IP assay was performed to explore the interaction between PTBP1 and TARDBP in WT and ZNF385A KO cells. Results are representative of two independent experiments. f The pie chart displaying the types and percentages of DNA regions for the retrotransposons enriched in the KO_dsRIP group. np, non- protein. pr, protein. CDS, coding sequence. UTR, untranslated Region. g The bar plot for the origins (intronic and non-intronic) of retrotransposons, including SINE, LINE, and LTR, enriched in ZNF385A KO_dsRIP samples. SINE, short interspersed nuclear element. LINE, long interspersed nuclear element. LTR, long terminal repeat. h Reads detected from CELF2 as a representative transcript containing intron-embedded retrotransposons. i–k J2-enriched retrotransposon-containing introns in CELF2 are predicted to form double-stranded secondary structures, including Alu (i), ERVL (j), and L1 (k). Source data are provided as a Source Data file.