Fig. 4: Gpat4 deletion elicited cGAS-STING-TBK1-IRF3-typ I interferon response in the endocardium. | Nature Communications

Fig. 4: Gpat4 deletion elicited cGAS-STING-TBK1-IRF3-typ I interferon response in the endocardium.

From: GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development

Fig. 4

A RNA-seq analysis of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts, Heatmap of dysregulated genes. Red and blue colors represent upregulated and downregulated genes, n = 3. B KEGG analysis of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts. C qPCR analysis of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts, n = 3. Left-Right, ***P = 0.0003, ***P = 0.0004, ***P = 0.0002, ***P < 0.0001, ***P = 0.0003, ***P = 0.0009, ***P = 0.0007, ***P = 0.0002, ***P = 0.0009, **P = 0.0057. D, E Western blotting analysis and quantification of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts, n = 3. Left-Right, **P = 0.0050, **P = 0.0086, **P = 0.0022, *P = 0.0453. F cGAS staining of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts. Scale bars, 100 µm. cGAS (in green), Endomucin (EMCN) stains endocardium (in red) and DAPI stains the nuclei (in blue). G co-localization analysis of cGAS and endocardium. H STING staining of Gpat4F/F and Tie2-Cre; Gpat4F/F E14.5 hearts. Scale bars, 100 µm. STING (in green), Endomucin (EMCN) stains endocardium (in red) and DAPI stains the nuclei (in blue). I co-localization analysis of STING and endocardium. Data are mean ± s.e.m. Two-tailed unpaired Student’s t-test.

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