Fig. 6: Blockage of the cGAS-STING-type I interferon response pathway restored endocardial development in Gpat4-deficient mice. | Nature Communications

Fig. 6: Blockage of the cGAS-STING-type I interferon response pathway restored endocardial development in Gpat4-deficient mice.

From: GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development

Fig. 6

A, B Gross analysis of embryos and hearts and quantification analysis of WT, Gpat4-/- and Gpat4-/-; Ifnar1-/- E14.5 hearts, n = 3. **P = 0.0046, **P = 0.0020. Scale bars, 500 µm. C, D Histological analysis of trabecular and myocardial layers, and quantification of WT, Gpat4-/- and Gpat4-/-; Ifnar1-/- E14.5 hearts, n = 3. ***P = 0.0006, ***P = 0.0005. Scale bars, 100 µm. E, F qPCR analysis of WT, Gpat4-/- and Gpat4-/-; Ifnar1-/- E14.5 hearts, n = 3. E Left-Right, **P = 0.0045, ***P < 0.0001, ***P = 0.0001, ns P = 0.1205, ***P < 0.0001, ns P = 0.5378, ***P = 0.0002, ns P = 0.6501, ***P = 0.0004, ns P = 0.6854, **P = 0.0011, ns P = 0.9643, **P = 0.0014, ns P = 0.0563, ***P = 0.0003, ns P = 0.6969. F Left-Right, ***P < 0.0001, **P = 0.0019, ***P = 0.0003, **P = 0.0025, **P = 0.0048, *P = 0.0175, **P = 0.0068, **P = 0.0048, **P = 0.0067, *P = 0.0325, ***P = 0.0007, **P = 0.0035, ***P = 0.0001, *P = 0.0113, **P = 0.0067, **P = 0.0063. G, H Western blotting analysis and quantification of WT, Gpat4-/- and Gpat4-/-; Sting-/- E14.5 hearts, n = 3. Left-Right, ***P < 0.0001, ns P = 0.1314, ***P < 0.0001, ***P < 0.0001, ***P = 0.0001, *P = 0.0178, *P = 0.0152, *P = 0.0269. I, J Gross analysis of embryos and hearts and quantification analysis of WT, Gpat4-/- and Gpat4-/-; Sting-/- E14.5 hearts, n = 3. *P = 0.0103, **P = 0.0352. Scale bars, 500 µm. K, L Histological analysis of trabecular and myocardial layers, and quantification of WT, Gpat4-/- and Gpat4-/-; Sting-/- E14.5 hearts, n = 3. *** P < 0.0001, *** P < 0.0001. Scale bars, 100 µm. M, N qPCR analysis of WT, Gpat4-/- and Gpat4-/-; Sting-/- E14.5 hearts, n = 3. M Left-Right, **P = 0.0038, ***P < 0.0001, ***P = 0.0003, ns P = 0.5043, ***P < 0.0001, ns P = 0.8883, ***P = 0.0002, ns P = 0.8013, ***P < 0.0001, ns P = 0.9270, ***P = 0.0006, ns P = 0.1899, ***P = 0.0003, ns P = 0.8434, ***P = 0.0001, ns P = 0.6958. N Left-Right, ***P = 0.0004, *P = 0.0207, ***P < 0.0001, ***P = 0.0004, **P = 0.0037, **P = 0.0084, **P = 0.0026, **P = 0.0050, **P = 0.0034, *P = 0.0208, **P = 0.0018, **P = 0.0095, ***P = 0.0001, *P = 0.0126, *P = 0.0139, *P = 0.0495. O, P Western blotting analysis and quantification of WT, Gpat4-/- and Gpat4-/-; Sting-/- E14.5 hearts, n = 3. Left-Right, ***P < 0.0001, ns P = 0.9582, ***P = 0.0009, ***P < 0.0001, ***P = 0.0002, ***P < 0.0001, ***P = 0.0009, *P = 0.0306. Data are mean ± s.e.m. Two-tailed unpaired Student’s t-test.

Back to article page