Fig. 1: Characterization of AZG-mediated adenine and trans-zeatin transport and binding.
From: Structural basis of cytokinin transport by the Arabidopsis AZG2
a Cell-based transport assay result showing that the uptake of [3H]-adenine was increased in HEK293F cells expressing AZG1 or AZG2, compared with control cells (transfected with the empty vector). b Cell-based transport assay result showing that the uptake of [3H]-trans-zeatin was increased in HEK293F cells expressing AZG1 or AZG2, compared with control cells. c Concentration-dependent [3H]-adenine uptake by AZG2. d Concentration-dependent [3H]-trans-zeatin uptake by AZG2. Data points in (c) and (d) were nonlinear-fitted using the Michaelis–Menten equation. All data points in (a–d) represent three (n = 3) independent measurements. Data are mean ± s.d. Significant differences in (a) and (b) were determined by one-way ANOVA with Dunnett’s multiple comparisons of the experimental groups against the control group. ns, not significant; * P < 0.0332; ** P = 0.0025; *** P < 0.0005; **** P < 0.0001. Exact P values are labeled. e ITC results for adenine binding to AZG2 at pH 5.5. The inset panel shows the original heat-change recordings. f ITC results for trans-zeatin binding to AZG2 at pH 5.5. The ITC experiments in (e) and (f) were each repeated three times with similar results. Source data is provided as a Source Data file.
