Fig. 4: Adenine and trans-zeatin coordination by AZG2.
From: Structural basis of cytokinin transport by the Arabidopsis AZG2
a Cartoon representation of the AZG2 structure in the adenine-bound state at pH 5.5. The two protomers are colored dark magenta and blue, respectively. The adenine molecules are shown in spheres, indicated by red dashed circles, with carbon atoms colored yellow and nitrogen atoms colored blue. b Coordination of adenine by AZG2 at pH 5.5. Side chains of residues surrounding adenine are shown in sticks, with carbon atoms colored purple. Hydrogen bonds are shown as red dashed lines. c Characterization of [3H]-adenine transport for the wild-type (WT) AZG2 and the mutants in the cell-based transport assay. d Cartoon representation of the AZG2 structure in the trans-zeatin-bound state at pH 5.5. The tZ molecules are shown in spheres, with carbon colored cyan, nitrogen colored blue, and oxygen colored red. e Coordination of trans-zeatin by AZG2 at pH 5.5. Side chains of residues surrounding trans-zeatin are shown in sticks, with carbon atoms colored purple. f Characterization of [3H]-trans-zeatin transport for the wild-type AZG2 and the mutants in the cell-based transport assay. All data points in (c) and (f) represent three (n = 3) independent measurements. Data are mean ± s.d. Significant differences were determined by one-way ANOVA with Dunnett’s multiple comparisons tests. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Exact P values are labeled. Source data is provided as a Source Data file.
