Fig. 2: YAP activation in hepatocytes alters BA composition in the liver and serum. | Nature Communications

Fig. 2: YAP activation in hepatocytes alters BA composition in the liver and serum.

From: Disrupting bile acid metabolism by suppressing Fxr causes hepatocellular carcinoma induced by YAP activation

Fig. 2

a Total BA concentration in the liver and serum of the TetO-YAP mice as measured by mass spectrometry, *P = 0.038, ****P < 0.0001. b Pie charts show percent composition of BA species in the liver and serum of the TetO-YAP mice. The color legend is located on the right side. c TCA, TβMCA concentration in the liver and serum of the TetO-YAP mice, *P = 0.041, **P = 0.0048, **P = 0.0067, ****P < 0.0001 in sequence. d Schematic diagram of the experimental procedure. e Total BA concentration in the liver and serum of the AAV-TBG-YAP * injected mice and paired control mice, as measured by mass spectrometry, ***P = 0.0005, **P = 0.002. f Pie charts showing the percent composition of BA species in the liver and serum of the AAV-TBG-YAPS127A injected mice and paired control mice. The color legend is the same as in (b). g TCA, TβMCA concentration in the liver and serum of the AAV-TBG-YAPS127A injected mice and paired control mice, **P = 0.0068, **P = 0.0032, *P = 0.026, *P = 0.014 in sequence. h Western blotting analysis of indicated genes in the mouse mature hepatocyte organoids (MHOs). i, j qRT-PCR analysis of indicated genes in the mouse MHOs. ****P < 0.0001 (i); **P = 0.0038, *P = 0.05 (j). k Western blotting analysis of indicated genes in the mouse MHOs. l, m qRT-PCR analysis of indicated genes in the mouse MHOs, ****P < 0.0001 (l); **P = 0.0045 (Shp, l); **P = 0.003, *P = 0.045 (Bsep, l); ****P < 0.0001, ***P = 0.0003 (Cyp7a1, m); ***P = 0.0006, **P = 0.0042, *P = 0.022 (Cyp8b1, m). data are presented as Mean ± SD, 1-way ANOVA with Sidak’s test. n = 3 biological replicates in Dox- group, n = 4 biological replicates in Dox+ 2weeks group (a–c), n = 3 biological replicates in each group (e, g), n = 3 independent experiments (i, j, l, m). Data are presented as Mean ± SD, p values were determined by two-sided unpaired t-test (a, c, e, g, i, j). Similar results were obtained from three independent experiments (h, k). Source data are provided as a Source Data file. Image in (d) was created in BioRender (Jin, Y. (2025) https://BioRender.com/h79u623).

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