Fig. 7: Fxr activation and HDAC1 inhibition suppress YAP-induced hepatic tumor formation.

a Schematic diagram of the experimental procedure. b Representative liver images in the indicated mice. c Liver/body weight ratios of the indicated mice, ****P < 0.0001, **P = 0.0023. d Serum ALT of the indicated mice, ****P < 0.0001, *P = 0.04. e Serum BA concentration of the indicated mice, ****P < 0.0001. *P = 0.026, *P = 0.047 in sequence. f–h qRT-PCR analysis of indicated genes in the liver tissue, ****P < 0.0001. **P = 0.017, **P = 0.023, ***P = 0.0003 in sequence (Ctgf, g);  **P = 0.0036 (Cyr61, g); ***P = 0.0003, **P = 0.0017,  *P = 0.049 (Col1a1, g); **P = 0.0089 (Acta2, g); ***P = 0.0003,  *P = 0.041 (Ccl2, h); **P = 0.001,  *P = 0.012 (Il1b, h); ***P = 0.0005 (Tnf, h). i Representative immunofluorescent images of the indicated liver samples. j Schematic diagram of the experimental procedure. k, l Intracellular (k), and medium (l) BA concentrations in hMHOs after treatment, ****P < 0.0001. ***P = 0.0002 (k); ***P = 0.0002, *P = 0.034, ***P = 0.0002 in sequence (l). m–o qRT-PCR analyses of hMHOs after treatment, ****P < 0.0001. **P = 0.0019, *P = 0.047 (CYP7A1, m); **P = 0.0087 (CYP8B1, m); **P = 0.0049 (CTGF, n); **P = 0.0013, **P = 0.0062 in sequence (CYR61, n); *P = 0.017 (SHP, o); *P = 0.0008 (BSEP, o). Scale bars: 1 cm (b), 100 μm (i). n = 5 biological replicates in each group (c–e), n = 3 biological replicates in each group (f–h), n = 3 independent experiments (k–o). Data are presented as Mean ± SD, p values were determined by 1-way ANOVA with Sidak’s test (c–h, k–o). Similar results were obtained from three independent experiments (i). Source data are provided as a Source Data file. Image in (a, j) was created in BioRender (Jin, Y. (2025) https://BioRender.com/h79u623).