Fig. 1: SPATA5, SPATA5L1, C1orf109 and CINP assemble into a stable complex and bind to the ribosomal particles.
From: Cryo-EM structure of the AAA+ SPATA5 complex and its role in human cytoplasmic pre-60S maturation
a Size-exclusion chromatography of the affinity-purified SPATA5 complex from cells co-expressing SPATA52EQ(E454Q/E728Q), C1orf109, SPATA5L1, and CINP, showing two separated peaks labeled as peak 1 and peak 2. b SDS-PAGE and silver staining analysis of the peak 1 sample, and coomassie blue staining analysis of the peak 2 samples. Protein bands corresponding to SPATA52EQ, C1orf109, SPATA5L1, and CINP are labeled. HSP70 is labeled with an asterisk. Source data are provided as a Source Data file. c, d Negative staining electron microscopy image of the peak 1 (c) and peak 2 (d) samples. e, f Ribosome profiling of the cell lysates co-expressing SPATA52EQ-Flag, HisSPATA5L1, StrepCINP, and HAC1orf109. Western blotting was used to confirm the presence of these proteins in the ribosomal fractions with Flag, His, Strep, and HA tag antibodies, respectively. Source data are provided as a Source Data file.
