Fig. 5: Structural features of the P-loop of the D2-ring of the SPATA5 complex.
From: Cryo-EM structure of the AAA+ SPATA5 complex and its role in human cytoplasmic pre-60S maturation
a–c The P-loops of SPATA5/SPATA5L1 D2 domains, with their densities shown. Only the SPATA5L1 D2 domain contains an ATP molecule. d–f The atomic models of the P-loops of the SPATA5/SPATA5L1 D2 domains showing the different conformations of the Cysteine residues in SPATA5 and SPATA5L1. g Sequence alignment of the P-loops of classical type II AAA+ ATPases, including human SPATA5, SPATA5L1, NSF, and p97, as well as yeast Drg1 and Cdc48, and Drosophila p97. h Structural alignments of the P-loops of SPATA5L1 D2 domain and SPATA51/SPATA52 D2 domains, demonstrating the steric clashes between the P-loops of SPATA51/SPATA52 D2 domain and ATP molecules.
