Fig. 6: Structure of the pre-60S particle bound with the SPATA5 complex.

a Cryo-EM map of the pre-60S particle co-purified with the mutant SPATA5 complex. Assembly factors TMA16, GTPBP4, eIF6, ZNF593, RLP24 and 5S RNA and 28S RNA H68-H69 are color-coded based on pdb:6LSS. b Representative particles of the pre-60S bound with the mutant SPATA5 complex from the negative staining EM. The position of the SPATA5 complex is annotated using the dashed circle. c Cryo-EM map of the pre-60S particle bound with the SPATA5 complex. Assembly factors GTPBP4, RLP24, PA2G4, the SPATA5 complex and 28S rRNA ES27A are color-coded. The two interfaces between the pre-60S particle and the SPATA5 complex are indicated with red arrows. The cartoon model of the SPATA5 complex illustrates its orientation relative to the pre-60S particle. d Cryo-EM map of the yeast pre-60S bound with Drg1 hexamer (EMD-14471). Assembly factors Nog1, Rlp24, Arx1, Drg1 complex, and the ES27 of 25S rRNA are color-coded. The interface between the pre-60S particle and the Drg1 complex is indicated with a red arrow. The cartoon model of the Drg1 complex illustrates its orientation relative to the pre-60S particle. e The CINP-GTPBP4 interface on the pre-60S particle. f The CINP-ES27A interface on the pre-60S particle. g The cross-linking analysis of the pre-60S-SPATA5 complex. The cross-linking between RLP24 with SPATA5, C1orf109, and CINP are indicated and colored blue, cyan, and lime, respectively. h Western blot analysis of input and GST IP samples probed with GST, Strep, or HA antibodies. Non-specific bands were labeled with an asterisk. Source data are provided as a Source Data file.