Fig. 7: Auto-sumoylation of Ubc9 alters the sumoylation of factors involved in translation and energy metabolism.

a Pie chart showing the distribution of SUMO-modified proteins in only wild-type (WT; magenta) and in both WT and ubc9-RR (green); no sumoylation proteins were identified only in ubc9-RR. SUMO-modified proteins were identified via mass spectrometry, as shown in Fig. 4a. b, c KEGG analysis of the SUMO-modified proteins found in only WT (b) and in both WT and ubc9-RR (c), as shown in Fig. 6g. Numbers indicate the number of proteins identified in each pathway. d, e Schematic diagram showing the list of Ubc9 auto-sumoylation-governed targets in the translation (d) and energy metabolism (e) pathways. Proteins in the two pathways depicted in b, c are marked as red and blue, respectively. Solid and dashed arrows denote the direction of fluxes and pathways in which intermediate molecules are not depicted, respectively. Metabolites in metabolic reactions are represented as yellow hexagons. The pie graph within d shows the distribution of SUMO targets among ribosomal subunits, and are classified by the indicated groups. PPP pentose phosphate pathway, DHAP dihydroxyacetone phosphate, PDH complex pyruvate dehydrogenase complex, Ac-CoA Acetyl-CoA, OAA oxaloacetate, ETC electron transport chain. Source data are provided as a Source Data file.