Fig. 6: Igf2bp3 is dispensable for normal hematopoiesis.

A Schematic outline of experimental strategy testing the effect of Igf2bp3 KO on mouse static normal hematopoiesis. n = 7 mice per group. B–D PB analysis of Igf2bp3 WT and KO mice. The density of white blood cells (WBC), lymphoma cells (LYM), red blood cells (RBC) (C), palates (PLT) (D), neutrophils (NEUT), monocytes (MONO), and eosinophils (EO) (E) are shown. E Flow cytometric gating strategies for HSC and progenitors. F, G Frequencies of various hematopoietic progenitors in the BM of Igf2bp3 WT and KO mice as examined by flow cytometry. LSK, Lin^-Sca1⁺cKit⁺ cells; MPP, multipotent progenitor (Lin^-Sca1⁺cKit⁺CD48⁺CD150^-); LT-HSC, long-term HSC (Lin^-Sca1⁺cKit⁺CD48^-CD150⁺); ST-HSC, short-term HSC (Lin^-Sca1⁺cKit⁺CD48^-CD150^-); LMPP, lymphoid-primed multipotent progenitor (Lin^-Sca1^highcKit^highCD127⁺CD135⁺); CLP, common lymphoid progenitor (Lin^-Sca1^lowcKit^lowCD127⁺CD135⁺); CMP, common myeloid progenitor (Lin^-Sca1^-cKit⁺CD34^intCD16/32^low); GMP, granulocyte-monocyte progenitor (Lin^-Sca1^-cKit⁺CD34⁺CD16/32^high); MEP, megakaryocyte–erythroid progenitor (Lin^-Sca1^-cKit⁺CD34^-CD16/32^-). H Flow cytometric gating strategies for mature cells. I Frequencies of various mature cells in the BM of Igf2bp3 WT and KO mice as examined by flow cytometry. J Schematic outline of competitive repopulation assay. n = 7 mice per group. K Flow cytometric gating strategy for BM cells derived from Igf2bp3 WT or KO donor mice in the competitive repopulation assay. L Flow cytometry analysis for different donor-derived cells (CD45⁺) in PB of recipient mice 4, 8, 12, and 16 weeks after BMT. M, N Percentage of donor-derived stem cell (M) and progenitor (N) compartments in the bone marrow of recipients 16 weeks after BMT. Mean ± SD values are shown. Statistical analysis: Two-tailed student’s t-test (B–D, F, G, I, L–N); n.s., not significant. Source data are provided as a Source Data file.