Fig. 2: Peritoneal-resident cavity macrophages are immunosuppressive by nature.

A Radarplot showing TAM median Ucell score signature per macrophage subset in PF. B Dot plot of selected genes encoding pro- (red) or anti-inflammatory (green) genes in all four monocytes + macrophages subsets. C Heatmap showing the row-scaled mean log₂transformed expression of top 5 unique marker proteins per monocytes + macrophages subset. D GP-WNN UMAP feature plots of reclustered mononocytes + macrophages showing log transformed expression of C1QA and SPP1 in healthy PF, colon, and liver tissue. E Boxplot analysis of monocytes + macrophages, C1Q⁺ CMs, and SPP1⁺ CMs comparing PF (n = 5 donors), colon (n = 5 donors), and liver (n = 5 donors). Box: 25th to 75th percentiles; line: median; whiskers: minimum and maximum values. Statistics: Mann–Whitney test, two-tailed. *p = 0.032, **p = 0.0079. UMAP Uniform Manifold Approximation and Projection, scRNA-seq single cell RNA sequencing, CITE-seq cellular indexing of transcriptomes and epitopes sequencing, HC healthy controls, PF peritoneal fluid, Mono-macs monocytes + macrophages, mono-CM monocyte-like cavity macrophage, CM cavity macrophage, RTM resident-tissue macrophages, Angio pro-angiogenic, IFN interferon-primed, INFLAM inflammatory-cytokine enriched, LA lipid-associated, Prolif proliferating, Reg immune regulatory, pro-inflam pro-inflammatory, anti-inflam anti-inflammatory. Only statistically significant differences are shown (*p ≤ 0.05; **p ≤ 0.01). Source data are provided as a Source Data file.