Fig. 4: Peritoneal-resident immunosuppressive macrophages infiltrate PMs. | Nature Communications

Fig. 4: Peritoneal-resident immunosuppressive macrophages infiltrate PMs.

From: Peritoneal resident macrophages constitute an immunosuppressive environment in peritoneal metastasized colorectal cancer

Fig. 4

A UMAP color coded for PMs from PM-CRC patients derived mono-mac subsets (n = 8 patients; 1,747 cells). B Boxplot of proportion of macrophage subsets out of total macrophages in PMs from PM-CRC patients (n = 8 patients). Box: 25th to 75th percentiles; line: median; whiskers: minimum and maximum values. Statistics: Mann–Whitney test, two-tailed. *p = 0.038, **p = 0.0019; 0.003, ***p = 0.0002. C Feature plot showing VSIG4 expression in PM derived monocytes + macrophages subsets. D UMAP color coded for monocytes or macrophages for HC (n = 5 patients) as well as the PM-CRC patients (n = 8 patients) of PBMC-, PF-, and, from PM-CRC only, PM-derived monocytes and macrophages (n = 17,930 cells) using Harmony for batch correction95. E UMAP color coded for tissue and disease state of monocytes and macrophages for HC (n = 5 patients) as well as the PM-CRC patients (n = 8 patients) of PBMC-, PF- and, from PM-CRC only, PM-derived monocytes and macrophages (n = 17,930 cells) using Harmony for batch correction95. F Dendrogram based on the Euclidian pairwise distances calculated between monocytes and macrophage samples obtained from HC (n = 5 patients) and PM-CRC patients (n = 8 patients). G Schematic overview of experimental set-up of PKH26 in vivo macrophage labeling (Created in BioRender. Vermeulen, J. (2025) https://BioRender.com/oi0rimk). H Gating strategy used to identify peritoneal PKH26+ macrophages: Plots are representative for their respective group. I Boxplot showing the percentage of PKH26+ F4/80+ macrophages in Spleen, PF, and PM, 2 weeks after tumor cell injection without (−, n = 1 mouse) or with (+, n = 6 mice) PKH26 linker injection. Box: 25th to 75th percentiles; line: median; whiskers: minimum and maximum values. UMAP Uniform Manifold Approximation and Projection, CMs cavity macrophages, mono-CMs monocyte-like cavity macrophages, mono-macs monocyte-macrophage, Spl spleen, PF peritoneal fluid, PM peritoneal metastasis, HC healthy controls, PM-CRC peritoneal metastasized colorectal cancer, i.p. intraperitoneal. (ns = non-significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001). All UMAPs in this figure were generated using only gene expression data. Source data are provided as a Source Data file.

Back to article page