Fig. 7: Assessment of HSV-1 infection and autophagic flux in microglia following autophagy activators treatment on the NVU model. | Nature Communications

Fig. 7: Assessment of HSV-1 infection and autophagic flux in microglia following autophagy activators treatment on the NVU model.

From: A microengineered 3D human neurovascular unit model to probe the neuropathogenesis of herpes simplex encephalitis

Fig. 7

a At 7 dpi, confocal micrographs showing the HSV-1 infection (red, immunostained for viral gG protein) in astrocytes/neurons and microglia after adding the indicated drugs (n = 3 for biological replicates; n = 3 for technical replicates). b TCID50 assay showing the antiviral effects of different drugs on the brain side (n = 3 for biological replicates; n = 3 for technical replicates). c Confocal micrographs showing autophagic flux in microglia with the addition of indicated drugs following HSV-1 infection at 3 dpi n = 3 for biological replicates; n = 3 for technical replicates. The red dots indicate autolysosome, and the yellow dots indicate autophagosome. d Quantification of autolysosome and autophagosome numbers in microglia of the HSV-1-infected chips after adding indicated drugs (n = 3 for biological replicates; n = 3 for technical replicates). Ten cells were quantified for each chip. Data are presented as the mean ± SD and were analyzed using a one-way ANOVA followed by the Bonferroni post hoc test.

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