Fig. 5: Protein expression of pulmonary fibrosis before and after treatment. | Nature Communications

Fig. 5: Protein expression of pulmonary fibrosis before and after treatment.

From: Targeted immunotherapy rescues pulmonary fibrosis by reducing activated fibroblasts and regulating alveolar cell profile

Fig. 5

A Proteomic strategies of fibrotic tissues at postoperative day 0, 14 and 28 after BLM administration and PCA analysis of three time instances in BLM mice (n = 4, 4, 4). B–D Protein clusters obtained from K-means algorithm and showed in heatmap, in which 4126 DEPs were enriched into 6 clusters. Cluster annotation obtained from GOBP results (B); Dot plot showing top-10 GOBP terms enriched in proteins of cluster 3 (C); and ECM relevant proteins (D) at BLM postoperative day 0, 7 and 14. E Proteomic strategies of saline or CD5/βLNP-FAPCAR 2.2 treatment for BLM mice. PCA analysis showed the significant difference between BLM+Saline and BLM + CD5/βLNP groups (n = 4, 4). F Dot plot showing the top-5 GOBP, GOCC and GOMF enriched terms of downregulated DEPs corresponding to Fig. (E). G Schematic diagram of fibroblast clearance and ECM stiffness regulation. H Representative immunofluorescence images of PATS (Red: KRT8; Green: Sftpc; Blue: DAPI) (Scar bar: 50 μm). I Dot plot of GO enrichment from upregulated DEPs corresponding to Fig. (E). J Network projection of epithelial polarization pathways. ‘n’ means independent biological replications. P-value determined by Fisher’s exact test (C, F, I) and one-way ANOVA (D). (DEP: differentially expressed protein; BP: biological process; CC: cellular component; MF: molecular function). Created in BioRender (A, E, G) 65.

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