Fig. 7: Plasticity of alveolar cell for lung regeneration. | Nature Communications

Fig. 7: Plasticity of alveolar cell for lung regeneration.

From: Targeted immunotherapy rescues pulmonary fibrosis by reducing activated fibroblasts and regulating alveolar cell profile

Fig. 7

A Study design for mouse lung single-cell sequencing (n = 2, independent biological replicates). B Uniform manifold approximation and projection (UMAP) plot for clusters and annotation of impartial captured lung cells. C, D A comparison of cluster components (BLM+Saline vs. BLM + CD5/βLNP-FAPCAR 2.2) (C) and sample distribution of significantly changed clusters (D). E UMAP embedding of fibroblast clusters. F A comparison of fibroblast cell clusters (BLM+Saline vs. BLM + CD5/βLNP-FAPCAR 2.2). G Expression projection of fibrotic and overactivated fibroblast marker genes on UMAP. H, I UMAP plot and annotation for re-clustered alveolar cells (H) and a comparison of cluster states (BLM+Saline vs. BLM + CD5/βLNP-FAPCAR 2.2) (I). J Dot plot showing representative markers for each alveolar cell cluster. K Expression projection of specific alveolar epithelial cell markers. L, M PAPCs marker genes in the entire pulmonary cell clusters (L) and highlighted PAPCs marker genes in UMAP plots that re-clustered with alveolar cells (M). N RNA velocity analysis for clusters including alveolar cells and PAPCs. (PAPCs: proliferating alveolar progenitor cells). Created in BioRender (A)65.

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