Fig. 8: Apoe+ macrophages and effector T cells reconstituted pulmonary immunity. | Nature Communications

Fig. 8: Apoe+ macrophages and effector T cells reconstituted pulmonary immunity.

From: Targeted immunotherapy rescues pulmonary fibrosis by reducing activated fibroblasts and regulating alveolar cell profile

Fig. 8

A UMAP plot for re-clustered macrophage clusters (n = 2, independent biological replicates). B Cell percentages in different clusters. C Highlighted marker genes of alveolar, fibrotic and lipid associated macrophages. D CellphoneDB analysis between macrophage cluster pairs. E The significantly changed receptor-ligand pairs in the cluster 1/3 communication. P-value determined by Bimod test. F, G Pseudotime (F) and RNA velocity (G) analysis for developmental rate of Apoe+ mono-macrophages. H–J UMAP plots for re-clustered T cell clusters (n = 2): clusters annotation (H), a comparison of two groups (BLM+Saline vs. BLM + CD5/βLNP-FAPCAR 2.2) (I) and characteristic gene expression (J). K KEGG enrichment results for a combination of clusters 0,1,6 and a combination of clusters 7,8,9. L, M Pseudotime (L) and Slingshot (M) analysis of differentiation pathways starting from Tn cells. N Expression level of inducible T-Cell costimulatory (Icos) and interleukin-17a (Il17a), which increased with developmental trajectories. (AM: alveolar macrophages; Mono-M: monocyte-derived macrophages; Tn: naïve T cell; DNT: double negative T cell; Tm: memory T cell; Tc: cytotoxic T cell; Th: T helper cell; Treg: regulatory T cells).

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