Fig. 5: Size exclusion chromatography (SEC) analysis of the recombinant hexon with different 100K mutants. | Nature Communications

Fig. 5: Size exclusion chromatography (SEC) analysis of the recombinant hexon with different 100K mutants.

From: Molecular mechanisms of the viral encoded chaperone 100K in capsid folding and assembly of adenovirus

Fig. 5

a SEC analysis of hexon co-expressed with ad2-100K, △657–805, △680–805, △693–805, △600–608(GS),△285–310(GS), △1–38, △1–93, and △1–135, respectively. SEC analysis of hexon mutant V50L-A51F-T53W co-expressed with ad2-100K mutant A251F-A254W-V255L, hexon mutant V50L-A51F-T53W co-expressed with ad2-100K, and hexon co-expressed with ad2-100K mutant A251F-A254W-V255L. The peaks of soluble aggregation, hexon trimer, and monomer are indicated by red, orange, and blue dash lines, respectively. b Representative negative staining images of the mature hexon (hexon trimer) peak fractions collected from SEC analysis of hexon co-expressed with ad2−100K, soluble aggregation of 100K and hexon, mature hexon (hexon trimer) peak fractions collected from SEC analysis of hexon mutant V50L-A51F-T53W co-expressed with ad2−100K, and hexon monomer peak fractions collected from SEC analysis of hexon co-expressed with ad2-100K mutant A251F-A254W-V255L. c Top Left: SEC analysis of hexon mutant V50L-A51F-T53W co-expressed with ad2-100K. Top Right: SDS-PAGE analysis of the sample for SEC and the peak fractions collected form SEC analysis of hexon mutant V50L-A51F-T53W co-expressed with ad2-100K. Bottom Left: SEC analysis of hexon co-expressed with ad2-100K mutant A251F-A254W-V255L. Bottom Right: Western blot (WB) analysis of the peak fractions collected form SEC analysis of hexon co-expressed with ad2-100K mutant A251F-A254W-V255L.

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