Fig. 1: snCED-seq for post-fixed tissues overview. | Nature Communications

Fig. 1: snCED-seq for post-fixed tissues overview.

From: snCED-seq: high-fidelity cryogenic enzymatic dissociation of nuclei for single-nucleus RNA-seq of FFPE tissues

Fig. 1

The workflow of snCED-seq for post-fixed tissues includes single nuclei isolation by CED and HED method with the snRandom-seq method used in this study. The steps from nucleus extraction to targeted sequencing are shown. In contrast to HED, the nuclei prepared with CED were morphologically intact without leakage of RNA molecular. Created in BioRender. Tu, D. (2025) https://BioRender.com/uq32z5z.

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