Fig. 1: Purification and biochemical characterization of the PSII-FCPII supercomplex. | Nature Communications

Fig. 1: Purification and biochemical characterization of the PSII-FCPII supercomplex.

From: Structure of a photosystem II-FCPII supercomplex from a haptophyte reveals a distinct antenna organization

Fig. 1

a Sucrose density gradient profile, with the lowest brown band corresponding to the PSII-FCPII supercomplex. b SDS-PAGE analysis of PSII-FCPII. Bands were identified by comparison with known PSII core complexes, and the FCPII bands were identified by N-terminal amino acid sequencing. This SDS-PAGE pattern was reproduced more than 3 times with independent samples. c Native PAGE profile of PSII-FCPII, with two main bands corresponding to PSII-12 FCPII and PSII-6 FCPII supercomplexes, respectively, which co-exist in the sample. After migration, the gel was stained with Coomassie blue to reveal the molecular markers. This migration pattern was obtained with 3 independent purifications. d Absorption spectrum of PSII-FCPII. e Low-temperature (77 K) fluorescence emission spectrum of PSII-FCPII. Uncropped photos and raw data of all these experiments are provided as a Source Data file.

Back to article page