Fig. 2: Overall structures of hTLR4/MD-2 and mTLR4/MD-2 ectodomain in complex with three different α,α- DLAMs.
From: Structural insight into TLR4/MD-2 activation by synthetic LPS mimetics with distinct binding modes
a Chemical structures of α,α-DLAMs and their EC50 values52 for the TLR4-mediated release of specific cytokines. b Size exclusion chromatography (SEC) (left) and native gel electrophoresis (right) of DLAMs or LPS-bound TLR4/MD-2 complex. The selected fractions for cryo-EM analysis (P2) are marked with the red square. SEC was performed three times for cryo-EM analysis (n = 3). Samples used for native gel electrophoresis were derived from the SEC experiment, and the gels were processed in parallel. The native gel electrophoresis experiment was repeated independently 2 times with similar results. Source data are provided as a Source Data file. c The cryo-EM maps of the h- and mTLR4/MD-2 in complex with DLAM5, DLAM3, or DLAM1. The colors of each component are indicated. d Side view of h- and mMD-2 (surface model) with bound DLAMs (spheres). GlcN portion of the DLAM and GlcN-linked lipid chains are colored blue; Man and Man-linked lipid chains are colored salmon.
