Fig. 2: Identification of the MTI1 locus by map-based cloning.
From: A nuclear-encoded endonuclease governs the paternal transmission of mitochondria in Cucumis plants
a The phenotypes of P3A (♀), MSC16 (♂) and the F1 progenies. Red arrows indicate the MSC progenies. Scale bars, 10 mm. b Positional cloning of the MTI1 gene in cucumber. Preliminary mapping of the MTI1 using BSA-seq, and fine mapping using KASP markers with 382 F2 plants. 30 genes in the candidate region and the MTI1 were successfully cloned with in silico analysis. c The genomic organization of the MTI1 (CsaV3_3G040940) gene. Blue boxes represent 5′ and 3′ UTRs, yellow boxes and black lines represent exons and introns, respectively. There is 1-bp deletion (Δ909) for P3A line. d Sequence alignment of the MTI1 between the parental lines MSC16 and P3A. The position of the 1-bp deletion (Δ909) in P3A caused frameshift, resulting a premature stop codon. The red asterisk indicates the premature STOP codon in P3A.
