Fig. 3: AT2-specific deletion of let-7 clusters induce spontaneous progressive chronic inflammation and ILD. | Nature Communications

Fig. 3: AT2-specific deletion of let-7 clusters induce spontaneous progressive chronic inflammation and ILD.

From: Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosis

Fig. 3

a, b, f, g Representative Masson’s trichrome-stained sections of lung lobes at 1-month iTAM (a, b) or 6-months (f, g) booster-iTAM. Scale bars: 2 mm upper panels; 50 μm lower panels (n = 16 mice per group). c Differential cell counts from BALF of mice is shown (n = 8, control; n = 8, let-7afdAT2; n = 7, let-7bc2AT2). Macrophages (Mac), Neutrophils (Neu). Ashcroft score was used to evaluate lung injury after 1 month iTAM (d) or 6 months booster iTAM (h). Samples per group: (1-month: n = 5, control; n = 5, let-7afdAT2; n = 4, let-7bc2AT2); (6-months: n = 10, control; n = 10, let-7afdAT2; n = 5, let-7bc2AT2). Data are mean ± s.e.m. P values were determined by Kruskal–Wallis with Dunn’s correction. Hydroxyproline levels from lungs of indicated groups of mice at 1-month iTAM (e) or 6-months booster iTAM (i). 1-month iTAM (n = 4, control; n = 4, let-7bc2AT2; n = 5, let-7afdAT2); 6-months of booster iTAM (4 mice per group). c, e, i Data are mean ± s.e.m. P values were obtained by one-way ANOVA with Tukey’s multiple comparison test. a–i Representative of three independent experiments. Not significant (NS). Source data are provided as a Source Data file.

Back to article page