Fig. 7: Absence of let-7afd impairs AT2 to AT1 differentiation. | Nature Communications

Fig. 7: Absence of let-7afd impairs AT2 to AT1 differentiation.

From: Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosis

Fig. 7

a Schematic representation of experimental design to examine the role of let-7afd in AT2 to AT1 differentiation in cultured AT2 organoids. Created in BioRender. King, K. (2025) https://BioRender.com/x04u478. b, d Representative immunostaining for ADI, AT1 markers and BACH1, EZH2 expression in let-7afd−/− vs control AT2 organoids grown as in panel (a). (n = 3 mice per group). Panel (b) shows KRT8 (green), DAPI (blue). Panel (d) shows AGER (green), SFTPC (red), DAPI (blue) top row panels; HOPX (green); LGALS3 (red); DAPI (blue) second row panels; BACH1 (red), KRT8 (green), DAPI (blue) third row panels; EZH2 (red), KRT8 (green), DAPI (blue) bottom row panels. Scale bars: 25 μm. Quantification of KRT8+ (c), AGER+ (e), HOPX+ (f), LGALS+ (g), SFTPC+ (h), BACH1+ (i), or EZH2+ (j) cells respectively in total DAPI cells (n = 3 mice per group). Data are mean ± s.e.m. P values were determined by unpaired two-tailed Student’s t test. Each circle represents a mouse. Representative of three independent experiments. k Line plots show smoothed relative expression of selected let-7 hub targets in the ADI to AT1 trajectory defined in published dataset12. Expression values were estimated by fitting a generalized additive model across pseudotime trajectory12. KRT8 peak is represented by a dashed line. Source data are provided as a Source Data file.

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