Fig. 9: The murine let-7 targetome is enriched in IPF “aberrant basaloid” (AB) cells.

a UCell Enrichment scores for the murine let-7 targetome in AT2, AB, and AT1 cells from control (Ctrl) or IPF lungs. b Broken circle plots indicate the total number and proportions of let-7 targets which are significantly upregulated (UP), downregulated (DOWN), or non-significant (NS) between Ctrl and IPF cells. Not present (n.p.) indicates gene targets missing in published dataset GSE136831¹². c Gene process enrichment analysis for upregulated let-7 targets in AB cells compared to control AT2 cells was computed with a one-tailed hypergeometric test and FDR correction using Metascape⁶⁰. d The Hallmark EMT pathway enrichment scores in control vs IPF AT2, AB and AT1 cell samples. e Dot plots show the relative expression of selected let-7 targets and identity markers in basal, AT2, AB, and AT1 cells from control and IPF patients. The size of each dot represents the percentage of cells in the cluster expressing the gene, while the color represents the mean expression of gene in cell cluster. f, g Consensus targets of BACH1 and EZH2 were evaluated for enrichment in AT2, AB and AT1 cells in control and IPF samples. High confidence targets (HCT)s. a, d, f, g Data are mean ± s.e.m half violin plots for cell values. P values were obtained with a one-way ANOVA with Sidak post-hoc test (AT2 Ctrl, n = 2655; AT2 IPF, n = 496; AB, n = 448; AT1 Ctrl, n = 502; AT1 IPF, n = 176). Expression data was derived from published scRNA-seq dataset GSE136831¹⁴. Source data are provided as a Source Data file.