Fig. 1: Misalignment on structural determination in preferential oriented datasets. | Nature Communications

Fig. 1: Misalignment on structural determination in preferential oriented datasets.

From: CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles

Fig. 1

a Pose distribution of Uni-HA-Syn (left) and PO-HA-Syn (right) datasets. b Comparison of density maps for the Uni-HA-Syn reconstruction (top), PO-HA-Syn reconstruction (middle), and PO-HA-Syn auto refinement (bottom). Density map quality metrics for each density map are shown to the right. The global half-maps FSC is plotted as a solid blue line, and the map-to-model FSC is shown as a dotted purple line. The green shaded area (bounded by dotted green lines) indicates the spread of directional resolution values, defined as the mean ±1 standard deviation (SD). The light coral region represents the histogram of directional FSCs. c Particle misalignments in PO-HA-Syn auto refinement. d Visualization of reprojection error between auto refinement estimates and ground-truth orientations for selected particles.

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